In this painting the protagonist is Judith, who is depicted after killing the Nebuchadnezzar’s army commander, Holofernes. In the half-light behind the heroine appears the handmaid, who is ready to wrap the severed head of the warrior and to bring it back to the city of Bethlehem, recognizable in the background.

It can be infered that Agostino Beltrano, like other contemporaries of his, knew the artworks by Massimo Stanzione. However, he added a moderate interest in a neo-Venetian and Rubensian way typical of Giovan Benedetto Castiglione and Pietro Novelli.